Learning about fundamentals of molecular life-sciences, from molecular cell biology to synthetic biology with a pan european biohacker team. Assemble novel plasmid vector and subsequently transform it back into E. coli. All genetic elements are naturally occurring in E. coli species and will merely be rearranged using synthetic biology technology. No new genes are introduced to the species. The modifications are thus called cis-genetic and are free to use outside of the laboratory in many countries.