LB Agar Petridish for Ecoli
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23.9.2015

15g Agar Agar / Liter LB medium
 
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Boilon Agar

1/4 L water
5g Agar Agar
1 Boillon cube
2 tsp Sugar

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Malz Agar

1 Teelöfel Biermalzextrakt
1 Teelöffel Malz
5g Agar Agar

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Boilon Flüssigmedium

1/4 L water
1/2 Boillon cube
1 tsp Sugar

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Malz Flüssigmedium

1/2 Teelöfel Biermalzextrakt
1/2 Teelöffel Malz
5g Agar Agar

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**** PH auf 8-9 mit Natronlösung *******


LB-medium (1 L):
 1. Dissolve 10 g tryptone (assortment of peptides ), 5 g yeast extract, and 10 g NaCl in 950 mL deionized water.
 2. Adjust the pH of the medium to 7.0 using 1N NaOH and bring volume up to 1 liter.
 3. Autoclave on liquid cycle for 20 min at 15 psi. Allow solution to cool to 55°C, and add antibiotic if needed (50µg / mL of Amp or Kan).
 4. Store at room temperature or +4°C.

LB agar-plates:
 1. Prepare LB medium as above, but add 15 g/L agar before autoclaving.
 2. After autoclaving, cool to approx. 55°C, add antibiotic (if needed), and pour into petridishes.
 3. Let harden, then invert and store at +4°C in the dark.